The peptides were then incubated at room temperature for at least 1 h and purified on a C18 reverse phase column (GraceCVydac) and H2O:acetonitrile gradient using ?KTA explorer 100 FPLC system (Amersham Pharmacia Biotech). to improve the performance with which TCRs check for antigen pMHC following the initial few molecular encounters possess happened. [TCRfree][pMHCfree]/[pMHC:TCR] and … Continue reading The peptides were then incubated at room temperature for at least 1 h and purified on a C18 reverse phase column (GraceCVydac) and H2O:acetonitrile gradient using ?KTA explorer 100 FPLC system (Amersham Pharmacia Biotech)
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